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Spectral flow cytometry analysis of patient PBMCs Aliquots of 510 10 6 peripheral blood mononuclear cells (PBMCs) cryopreserved in fetal calf serum/ dimethylsulfoxide solution were defrosted, washed and resuspended in FACS Buffer (Brilliant Stain Buffer Plus BD + 5% fetal calf serum + 2 mM EDTA)