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Chemiluminescent signal was developed for 60 minutes at room temperature and quantified using an EnVision plate reader

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Manufacturing and Quality Both formulations share identical manufacturing and quality frameworks: All peptides manufactured via Solid-Phase Peptide Synthesis (SPPS) All peptides use synthetic amino acids no animal-derived materials All peptides independently tested by Janoshik Analytical 99% HPLC purity per peptide Mass spectrometry identity confirmation per peptide COAs published at durhampeptides.ca/lab-results There's no quality difference between the two formulations