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In the nigrostriatal tract system, dopamine undergoes autooxidation or oxidative deamination by monoamine oxidase-B, both of which aggravates with age, leading to increased production of peroxides and hydroxyl radicals 41
Following centrifugation, the supernatant was incubated with 50 l Strep-Tactin Sepharose resin, MBP resin, or GST beads (Beyotime, P2253) at 4 C for 1 h
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The absolute loss is still real, and still worth preventing, but the framing matters [7, 8]
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