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For the colorectal cancer PDX study, female NCR nude mice (CrTac:NCr- Foxn1 nu from Taconic) were implanted subcutaneously with low-passage KRAS-mutant CRM 13180 tumour fragments (30 mg) into the region of the right axilla
doi:10.1016/j.lfs.2011.01.015 Sikiric P, Seiwerth S, Rucman R, et al
The D-amino acid substitution preserves the spatial orientation of amino acid side chains needed for competitive binding to the FOXO4-p53 interaction surface, maintaining biological activity despite the non-natural backbone (PMID: 28340339)
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