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After blocking with 10% milk in 0.1% TBST for 1 h, the membrane was incubated with a primary antibody overnight at 4 C or 3-4 h at room temperature, followed by incubation with a secondary antibody for 1 h at room temperature

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A larger reconstitution volume or higher per-dose research unit consumes the vial faster and avoids the shelf-life issue

This multifactorial assault on glutathione homeostasis creates a compelling rationale for exogenous support, particularly through delivery methods that ensure reliable tissue concentrations