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To further explore this relationship, we conducted animal studies
Additionally, various molecular techniques, including Western blotting, reverse transcription, quantitative PCR, immunofluorescence, and immunohistochemistry, were utilized to examine the expression levels of angiogenesis-related factors and the Notch/NF-B pathways-related factors
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Morphine has been linked to highly selective mu-opiate receptor signaling, especially via truncated receptor variants associated with nitric oxide release, and this signaling has repeatedly been interpreted as down-regulatory, stabilizing, and protective in immune, vascular, neural, and progenitor cell systems [10,11]
