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When the fluorescent secondary antibody method was used, the sections were treated with Alexa 488- or Alexa 555-conjugated anti-mouse/rabbit IgG (1:500 dilution, Molecular Probes, Eugene, OR)
For upper arm injections, choose an injection site between the shoulder and elbow
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Emerging technologies, including brain organoids and microfluidic organ-on-chip platforms, provide enhanced physiological relevance by simulating multicellular brain environments and nutrient flow (Castiglione et al., 2022)
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