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The physical stress of ice crystal formation and thawing is too much for these intricate molecules
Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Biowest, Nuaille, France), 100 g/mL streptomycin, and 100 U/mL penicillin (Thermo Fisher Scientific) at 37C in a humidified atmosphere containing 5% CO 2
Upregulation of Antioxidant and Repair Genes: It increases the expression of genes responsible for DNA repair, antioxidant production (e.g., superoxide dismutase), and the synthesis of structural proteins like collagen and elastin
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