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The absorbance was measured at 517 nm using a Biotek Synergy HT multi-detection microplate reader (BioTek, Charlotte, VT, USA)

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DsbASP was oxidized with a 10-fold molar excess of AT-2 and roGFP2 was reduced with a 10-fold molar excess of DTT for 10 min at 20 C and then the oxidant or reductant were removed using Micro Bio-Spin P-30 gel columns (BioRad, Feldkirchen, Germany, #7326223) or NAP-5 columns (Cytiva, Freiburg, Germany, #17085302) according to the suppliers protocol

If irritation or rash occurs, discontinue use