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Outer PCR (30 l): 10X PCR buffer 3 l 2.5 mM dNTP 2 l, dH2O 17.6 l, primers (10 pmol/l) 1.5 l, Taq enzyme (2.5 U/l) 0.4 l, template cDNA 4 l
G protein-coupled receptors as new therapeutic targets for type 2 diabetes
Novel Peptide Treatment May Help to Prevent and Reverse Type 1 Diabetes Type 1 diabetes is an immune-mediated disorder that causes dysfunction of insulin-producing beta cells in the pancreas, leading to a defect in insulin production
The protein was expressed in Sf9 insect cells (Invitrogen) using the Bac-to-Bac baculovirus expression system (ThermoFisher)
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