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However, this shift is accompanied by a significant downregulation of gluconeogenic enzyme expression, resulting in hypoglycemia and lactate accumulation, which have been associated with increased severity of AKI and higher patient mortality (Legouis et al., 2020
Proc Natl Acad Sci USA 120:e2217864120
The cells were washed once in with 50 L PBS (Gibco, #14190-094) after which 30 L of each concentration from the dilution series was added to the 384-well assay plate with cells
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