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Therefore, there are multiple factors to consider when outlining the biggest differences between the two melanocortin agonists: Molecular Structure: While both MT1 and MT2 are synthetic analogs of alpha-MSH, they have only four amino acids in common: the sequence His-D-Phe-Arg-Trp (positions three to six in MT2)
They were mixed thoroughly and incubated for 10 min at room temperature
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