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The cows were fed a partial mixed ration according to the guidelines of the Society of Nutritional Physiology (GfE, 2001) for nutrient and energy supply, consisting of 50% roughage (70% maize silage and 30% grass silage) administered in feed-weigh troughs (Roughage Intake Control, System Insentec B.V., Marknesse, The Netherlands) and 50% concentrate feed, administered in electronic feeding stations (Insentec B.V., Marknesse, The Netherlands)

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Gut hormone analysis Total GLP-1 and PYY levels in cell supernatants and lysates, and in plasma, were measured using sensitive and specific in-house radioimmunoassays as previously described