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After being rinsed with PBS, the sections were treated with 3% H 2 O 2 for 15 min to quench endogenous peroxidase activity, permeabilized and blocked with 0.3% Triton X-100 in PBS containing 5% BSA for 1.5 h at room temperature

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This balanced regulation of both proteolytic and synthetic pathways suggests GHK-Cu peptide may act as a broader orchestrator of extracellular matrix homeostasis rather than a simple anabolic agent

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