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In brief, MSCs were re-plated at a clonal density of 60 cells/well in six-well culture plates and cultured in hES-MSC medium for 14 days
10.1038/ni.1675 27
If your vial size or water volume ever differs from this protocol, do not eyeball it run the exact figures through the dosage calculator, which does the concentration volume units conversion for any inputs
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Discussion The enzymatic formation and reduction of disulfides in physiological systems clearly differs from uncatalyzed redox equilibrations in vitro 5,7,63,78,79
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