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Key Takeaways Vitamin B12 is a crucial cofactor in DNA synthesis, energy production, and myelin synthesis
Total GSH is measured at an absorbance of 412 nm as the oxidized GSSG is reduced to two GSHs by NADPH, Glutathione reductase, and the reduced GSH and GSH present in the existing sample react with DTNB to form yellow TNB
However, subcutaneous injection may result in different tissue-level concentrations compared to oral supplementation, and even small amounts of NAD+ precursor could have local effects in adipose tissue near the injection site
Liss, Inc, 697-745, 1990
