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On day 7 seedlings were transferred to fresh growth medium, and then some of the plates were supplemented with AITC to a final concentration of 1 mM whereas others remained non-treated (control)

Seleninic or selenol intermediates generated from the oxidation/reduction of DSePA can follow the analogous catalytic steps as discussed for the native GPx enzyme in the earlier section to detoxify the hyperoxides by consuming the thiols [82, 83]

For researchers drawing from a reconstituted vial over days or weeks, this protection is essential