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In vitro BP incorporation was performed at 37 C for 1 h, and then, the samples were diluted with sample loading buffer (60 mM Tris-Cl (pH 6.8), 10% glycerol, 2% SDS, 1% -mercaptoethanol (ME), 0.01% bromophenol blue) and boiled at 100 C for 10 min

The BPC-157 anti-inflammatory effects observed in preclinical research appear to modulate the inflammatory environment in ways that support rather than suppress the reparative cascade, positioning it as a pro-healing agent rather than simply a pain management tool

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