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Abbreviations ELISA: Enzyme-linked immunosorbent assay IgG: Immunoglobulin G HRP: Horseradish peroxidase enzyme label O.D.: Optical density PBS: Phosphate buffer saline FBS: Fetal bovine serum Ab-c: Capturing antibody Ab-d: Detection antibody References Karen L, Viswanath D, Aidas K et al (2004) (2012, updated in 2019) Assay Guidance Manual