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Cells were then allowed to recover for 24 h in fresh medium before antibiotic selection with 2 g ml 1 puromycin for at least 4 days, after which they were expanded or used for subsequent experiments

In mouse models of highfat dietinduced liver steatosis, it significantly inhibits hepatic NNMT activity, reduces NAM methylation, increases NAD+ and SAM levels, enhances mitochondrial fatty acid oxidation, reduces hepatic triglyceride and lipid accumulation, lowers lipotoxicity markers (e.g., malondialdehyde, transaminases), improves hepatocellular injury, and reverses steatosis

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