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The pellet was resuspended in 2 ml RPMI-MGIA, adjusting splenocyte concentration to 3x10 6 or 5x10 6 in 600l for co-culture with BCG (300 or 500 CFU) in 48-well plates, and incubated for 96 hours at 37C with CO 2
In conclusion, the activation of GSTs together with other host genes encoding antioxidative and defense enzymes has been often observed during plant-symbiotic microbe interactions that resulted in enhanced resistance against microbial pathogens
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