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Slows the rate at which food leaves the stomach, prolonging feelings of fullness after meals and reducing overall food intake
Reverse transcription and real-time quantitative PCR (Q-PCR) Total RNA was isolated from frozen liver pieces using Tri-Reagent (Molecular Research Center, Inc.) according to manufacturers protocol
( Bmal1 and Clock directly to downregulate their transcription ( The other two available studies (
The resulting trimmed and de-duplicated reads were then aligned to the Rattus norvegicus Rnor6.0 reference genome, available on ENSEMBL, using STAR aligner v.2.5.2b4 25 , producing BAM files
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