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DNA fragments of ER-shRNA#30, ER-shRNA#98, SLC7A11-shRNA#3, SLC7A11-shRNA#24, SLC3A2-shRNA#86 and SLC3A2-shRNA#87 (sequences from Sigma-Aldrich website) were subcloned into the Eco R I and Age I digested pLKO.1 vector, respectively

Ipamorelin was able to stimulate GH secretion in a dose-dependent manner, with Cmax values ranging from 20223 mU/l and AUC values from 30373 mU*h/l

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Interestingly, a recent study showed that oral supplementation of NR altered the intestinal microbial composition in rats and mice but not humans 63 , highlighting the need for further studies on this matter to assess the impact of NAD + metabolism modulation by the microbiome on health outcomes