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Creation of Super HGF: When HGF is treated with lipoic acid trisulfide (LASSS) at an 1:8000 molar ratio, its binding affinity for c-met increases more than two-fold compared to unnitrated, native HGF while remaining highly resistant to nitration
Because IGF-1 LR3 is a folded protein of roughly 9.1 kDa carrying three disulfide bridges rather than a short synthetic peptide, agitation is the main practical hazard: shear and air-liquid interface exposure drive aggregation, so swirl only and never shake
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