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Overexpression of TCF7L2 in HAEC using TCF7L2-EGFP adenovirus TCF7L2-EGFP adenovirus was prepared as described previously 24

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In this model, choline-deficient diet from 3 to 12 months of age disrupted liver and heart normal function, and altered neural networks associated with microtubule stability and postsynaptic membrane regulation in the hippocampus, elevated soluble and insoluble Amyloid- levels, increased Thioflavin S structures, and tau hyperphosphorylation at various pathological epitopes in the hippocampus and cortex (163)