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The samples were allowed to thaw briefly and then lysed in ice-cold RIPA buffer (Tris-HCl 50 mM, pH 7.4, sodium chloride 150 mM, 1% Nonidet P-40, 1% sodium deoxycholate, 0.1% SDS, 2 mM EDTA, 0.5 mM AEBSF (Santa Cruz, sc-202041), 25 mM beta-glycerophosphate (Sigma, G5422), 1 mM sodium orthovanadate (BioShop, SOV664), and 10 mM sodium fluoride (Sigma, S7920)) by rotation at 4 C for 30 min
Identify Objective Deficiencies Gordon emphasizes broad testing rather than symptom-based guessing
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These findings underscore the notable tissue-specific and context-dependent nature of histone Kla function
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