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Similar biphasic kinetics, although a 100-fold slower, were reported for the GSSG-dependent oxidation of fusion constructs between rxYFP and a mono- or dithiol variant of ScGrx1 from yeast 31
Marks MS, Seabra MC
After the plasmid was synthesized, PCR was used to confirm that the target gene of the fusion protein was synthesized correctly, and the upstream and downstream primers were designed F: 5'-TAATACGACTCACTATAGGG-3'R: 5'-GCTAGTTATTGCTCAGCGG-3'
And also looking at some animal data, and I will say like weak, pretty weak data at the time
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