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Western immunoblot analyses For the preparation of total liver homogenates, frozen liver pieces were homogenized in RIPA buffer (150 mM NaCl, 1% TritonX-100, 0.25% sodium deoxycholate, 0.1% SDS, 50 mM Tris, 1 mM EDTA, 1 mM PMSF, protease inhibitor cocktail, pH 7.4) on ice with a tissue tearor (BioSpec Products, Bartlesville)

Studies show curcumin may also help modulate glutathione enzymes (12)

pylori infection directly causes oxidative stress in gastric epithelial cells by the production of ROS, and it also stimulates host responses that result in ROS and controls the production of proinflammatory cytokines, inflammation, and cell death (99)