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Further, fibrotic ECM is known to contribute to the increased stiffness of fibrotic tissues, and this stiffness is essential for sustained TGF responses as stiff ECM induces mechanical strain and can help position latent TGF near MMPs, facilitating the liberation of active TGF (31, 249)

To knockdown GCL by small hairpin RNA (shRNA), a vector-based shRNA approach using the targeting sequence 5-GAAGGAGGCTACTTCTATA-3, as we described 5 , was used

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