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Many of the previous studies had methodological limitations such as selection bias [10, 12], and limited generalizability [11, 29]
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Peak weight loss acceleration occurs during the dose-titration phase (weeks 4-20), with clinical trial data showing an average loss of 15-22% of baseline body weight by week 20
Experiments were conducted in a box (35 cm length 25 cm width 25 cm height), with the box wiped clean with alcohol after each observation to remove feces and urine
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