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For the isolation of PBMCs, blood from the same donor was pooled and diluted with an equal volume of PBMC isolation buffer containing 0.1% bovine serum albumin (Carl Roth, Karlsruhe, Germany) and 2 mM ethylenediaminetetraacetic acid (EDTA) (Sigma-Aldrich) in phosphate buffered saline (PBS) without calcium and magnesium (Lonza, Cologne, Germany)
The RNA-small molecule mixture was incubated at 37 C for 60 min
Additionally, it may not be appropriate for people with certain other medical conditions
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