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However, the impact of combining these therapies remains under-researched

This pattern mirrors what occurs with other weight loss interventions, including many forms of bariatric surgery and intensive lifestyle modification, when ongoing support is not maintained

2 Materials and methods 2.1 Animal studies Seipin knockout (SKO) mice with germline disruption of the Bscl2 gene were generated as previously described on a C57BL/6J background (19)