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However, overtraining without adequate rest can actually deplete glutathione

The damage from freeze-thaw cycles comes from repeated freezing and thawing of the same solution, where ice crystals form, melt, reform, and melt again, each cycle physically stressing the peptide molecules

Twenty microliters of supernatant was transferred into a 96-well plate, and 200 l of 37 C o-dianisidine hydrochloride solution was added immediately before the optical density was read at 450 nm and again 30 s later, as previously described 26

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