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Further, biomarker concentrations are tricky to interpret: Total GSH concentration often fails to accurately reflect redox balance, which is better indicated by the GSH to GSSG ratio, plasma/serum concentrations are often a weak correlate of tissue level (the locus of action), and compartmentalization within cells (cytosol versus mitochondria) adds another layer of complexity often out of grasp in clinical samples [31]

45 Liver cytochrome P450 (CYP450) enzymes regulate the biotransformation of targeted agents (e.g., imatinib, erlotinib), and polymorphisms in these enzymes can result in therapeutic failure or toxicity