glp-1 and microbiome Bidirectional Interactions Between the Gut Microbiota Incretin-Based Therapies EP. 172: GLP-1 Agonists and

Dec 2020;23(12):1501-1526

The colony regions were defined by applying an automated thresholding algorithm (Otsu method)

[9] Kalra S, Kalra B, Kumar S, Sharma A

Parameterized Framework Inputs: concentration_mcg_per_mL peptide concentration after reconstitution (or convert: mg/mL 1000 = mcg/mL) pump_output_mL_per_actuation delivered volume per spray (from manufacturer specs or direct measurement) Output: amount_mcg_per_actuation = concentration_mcg_per_mL pump_output_mL_per_actuation Optional Volume Mapping (U-100 Convention) If units are used as a volume shorthand (where 100 units = 1.0 mL on a U-100 scale): pump_output_mL_per_actuation = units_per_actuation / 100 amount_mcg_per_actuation = concentration_mcg_per_mL ( units_per_actuation / 100) Nasal Actuation Calculation Table (Placeholders) Worked Examples (Placeholders Only) Example 1 (mL-based pump): If concentration = 3333 mcg/mL (i.e., 10 mg reconstituted in 3.0 mL) and pump output = 0.10 mL/actuation , then amount per actuation = 3333 0.10 = 333.3 mcg/spray