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Mechanistic target of rapamycin complex 1 (mTORC1) detects the concentration of cysteine via the small G protein Recombination activating gene (Rag) and positively regulates the translation and expression of GPX4 protein by phosphorylating eukaryotic initiation factor 4E-binding proteins (4EBPs)
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This violates the "Original" component of ALCOA and the explicit requirement of 211.68(b) that "A backup file of data entered into the computer or related system shall be maintained." The FDA considers the electronic stream from the instrument to be the raw data
