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Upon blood collection, liver, kidney, gill, and heart samples were immediately and meticulously excised, rinsed with physiological saline solution (0.9% NaCl), and stored at 80C until biochemical analysis, conducted within one month of extraction

10.4 How are peptides administered

In addition, we have observed increased neuronal activation in the LH upon OB GLP-1R stimulation and the existence of an anatomical connection between the AON and LH