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Perifusate fractions were collected every minute for 45 min, and insulin concentrations were quantified using an ELISA kit (ALPCO, Salem, NH, USA) to evaluate basal and glucose-stimulated insulin secretion
L.SebastianiG.GriecoF
Repeating the loading dose may be considered in patients undergoing hemodialysis
a 50 mcg dose at that concentration is 0.10 mL (10 units on a U-100 insulin syringe)
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