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doi: 10.1016/j.cell.2015.11.055 57 JiaJDouPGaoMKongXLiCLiuZet al

The goal is sustainable, healthy weight reduction rather than maximum speed of loss, which can help minimise adverse effects including cold sensitivity

They have suggested, based on biophysical characterization and native polyacrylamide gel electrophoresis (PAGE) analysis, that EcGGT dissociated into individual subunits upon heat treatment, which reassembled into active conformation at 4C (Ho et al., 2013)