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Then, 15 g of protein was mixed with NativePAGE sample buffer (Invitrogen), Coomassie G-250 and protease inhibitor, loaded into the lanes of a 312% Bis-Tris NativePAGE gel (Invitrogen) and run according to the manufacturers instructions
Alsina-Beauchamp D, Escs A, Fajardo P, Gonzlez-Romero D, Daz-Mora E, Risco A, Martn-Serrano M, Del-Fresno C, Dominguez-Andrs J, Aparicio N, Zur R, Shpiro N, Brown G, Ardavn C, Netea M, Alemany S, Sanz-Ezquerro J, Cuenda A (2018) Myeloid cell deficiency of p38/p38 protects against candidiasis and regulates antifungal immunity