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The compounds are supplied together in one unit, so it is a single reconstitution step
As a fifth example, the amino group of the N-terminal amino acid has a predominant positive net charge below pH~8.0, thus for cation exchange the pH elution gradient with a solvent comprising an organic modifier could begin below pH 8.0 to remove a form extended with an undesired acyl group and end the gradient above pH 8.0 thereby subsequently eluting the target GLP-1 moiety
P = 0.253)
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