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Although these proteases retained the ability to cleave procaspase-1 in co-transfected HEK293T cells, albeit at a reduced capacity compared to X(PC), their activity was substantially diminished in the THP-1 system
For example, Pollak and colleagues 59 reported an NADPH:NADP + ratio of ~1:4 using an HPLC-based approach on HEK293 cell lysate which would be fully consistent with the NAPstar measurements
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