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There is no report of its occurrence in archaea which indicates that melatonin originated in bacteria [11] most likely to prevent the first cells from the damaging effects of oxygen in the primitive Earth's atmosphere

Beyond 48 hours, potency loss becomes measurable

After deparaffinization, sections were heated in an autoclave at 121 C for 5 min in 10 mM sodium citrate buffer (pH 6.0) or reacted with protease solution (Nichirei Biosciences, Tokyo, Japan) for epitope retrieval, after which endogenous peroxidase was inactivated by 0.3% hydrogen peroxide in methanol