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To construct the sorting vector for gene overexpression, we amplified the whole expression cassette of N-signal-GST-EGFP-GPI DAF and N-signal-GST-EGFP-GPI CEAM7 from pEGFP-GST-GPI DAF and pEGFP-GST-GPI CEAM7 vectors and joined them with PCR linearized pcDNA3.1 vector using ClonExpress II One Step Cloning Kit
This helps to prevent the cellular and tissue damage strongly associated with accelerated aging
Both aim to accelerate healing, reduce recovery time, and help the body repair damaged tissue
For example, staff can handle education on injection technique or side effect expectations
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