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For the tube formation assay, 50 L of Matrigel (Corning, Glendale, AZ, USA) was loaded into each well of a 96-well plate, and the plate was incubated at 37C for 30 min before culture

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Increased TMAO levels caused by high carnitine intake also impair liver function, highlighting the complex and fine regulatory role of carnitine in gutliver communication [248, 249]

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