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Cells were collected and sonicated using a Bioruptor UCD-300 (Cosmo Bio, Tokyo, Japan) in RIPA buffer containing inhibitor cocktails for proteases and phosphatases (Cayman Chemical, Ann Arbor, MI, USA)

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When GSH was tested together with reduced PfGrx C88S or PfGrx C32S/C88S , the syringe of the stopped-flow spectrofluorometer also contained 0.4 U/mL glutathione reductase (GR) and 0.25 mM NADPH to ensure the removal of trace amounts of GSSG that might interfere with the redox measurements