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The Ni-NTA resin was packed into a glass column and washed with 25 column volumes of 20 mM HEPES pH 7.4, 100 mM NaCl, 2 mM MgCl 2 , 0.01% (w/v) LMNG and 0.0006% (w/v) CHS, 1 M peptide, followed by elution using the same buffer supplemented with 250 mM imidazole and 3 mM CaCl 2

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Flow cytometry was further applied to observe the cellular uptake of nanoparticles

Among the issues were inconsistent naming, missing or inadequate descriptions of physical and chemical properties, incomplete impurity information, limited analytical detail, and uncertainty about how marketed materials correspond to the nominated substances