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KLOW is a compound blend typically containing: BPC-157 (Body Protection Compound 157) 300500mcg per dose TB-500 (Thymosin Beta-4 or TB4-Frag 1723) 22.5mg per dose GHK-Cu (Copper peptide GHK-Cu) 12mg per dose Some formulations also include small amounts of additional compounds (NAD+, KPV, or others), but BPC-157 + TB-500 + GHK-Cu is the standard KLOW core
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Then, the blots were incubated with the following antibodies overnight at 4 C: mouse anti-GLP-1R (1:200, Santa Cruz, CA, USA), mouse anti-GLP-1 (1:100, Santa Cruz, CA, USA), mouse anti-CGRP (1:100, Santa Cruz, CA, USA), mouse anti-c-fos (1:200, Santa Cruz, CA, USA), rabbit anti-Iba-1 (1:1000, Abcam, Cambridge, MA, USA), rabbit anti-PI3K (1:1000, Proteintech, China), rabbit anti-Akt (1:1000, Proteintech, China), rabbit anti-phospho-Akt (1:2000, Cell Signalling, Boston, MA, USA), rabbit anti-IL-1 (1:800, Wanleibio, China), rabbit anti-TNF- (1:800, Wanleibio, China), and mouse anti--actin (1:1000, Beyotime, China)
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