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You can continue using the same vial through your dose increases, discarding only when the 28-day mark hits or when you have used the full 5mg
The chromatin was subsequently immunoprecipitated with specific antibodies (RNA poly II and H3K27ac were positive control, NIgG was the negative control) against the target protein and incubated overnight at 4 C with gentle rotation
Institutional Review Board Statement: Not applicable
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