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The rats were kept in a 25C room with a 12-hour light/dark cycle, free access to water, and a standard diet from the Experimental and Clinical Research Center at Erciyes University in Kayseri, Turkey
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Log-based cultivation was the first step in growing wild shiitake on suitable wooden logs, and fruiting could be induced by providing a physical shock to the infected logs [10, 11]
Cefali LC, Ataide JA, Moriel P, Foglio MA, Mazzola PG
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